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Rabbit anti-NF1 Antibody, Affinity Purified

Catalogue number:
A300-140A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Neurofibromin immobilized on solid support. The epitope recognized by A300-140A maps to a region between residue 2760 and the C-terminus (residue 2818) of human Neurofibromin 1 using the numbering given in entry NP_000258.1 (GeneID 4763) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

neurofibromatosis 1, neurofibromatosis-related protein NF-1, neurofibromin, NFNS, truncated neurofibromin 1, VRNF, WSS

Species Reactivity:

Human, Mouse

Immunogen:

between 2760 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse NF1 by west

Detection of human and mouse NF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A300-140A (lot A300-140A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human NF1 by western blot o

Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A300-140A (lot A300-140A-3) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibody BL954. For blotting immunoprecipitated NF1, A300-140A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.