Rabbit anti-NF1 Antibody, Affinity Purified - A300-140A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Neurofibromin immobilized on solid support. The epitope recognized by A300-140A maps to a region between residue 2760 and the C-terminus (residue 2818) of human Neurofibromin 1 using the numbering given in entry NP_000258.1 (GeneID 4763) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human and mouse NF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A300-140A (lot A300-140A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A300-140A (lot A300-140A-3) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibody BL954. For blotting immunoprecipitated NF1, A300-140A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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