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Rabbit anti-ATR Antibody, Affinity Purified - A300-137A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ATR Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-137A
Name:
Rabbit anti-ATR Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ATR immobilized on solid support. The epitope recognized by A300-137A maps to a region between residues 400 and 450 of human Ataxia Telangiectasia and Rad3-related using the numbering given in entry NP_001175.1 (GeneID 545).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
ataxia telangiectasia and Rad3-related protein, FCTCS, FRAP-related protein 1, FRAP-related protein-1, FRP1, MEC1, MEC1, mitosis entry checkpoint 1, homolog, SCKL, SCKL1, serine/threonine-protein kinase ATR
Species Reactivity:
Human
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ATR by western blot. Samples: Whole cell lysate (50, 15 and 5 µg) from HeLa and (50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody A300-137A (lot A300-137A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ATR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATR antibody A300-137A (lot A300-137A-2) used for IP at 6 µg per reaction. ATR was also immunoprecipitated by rabbit anti-ATR antibody A300-138A. For blotting immunoprecipitated ATR, A300-137A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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