Antibody was affinity purified using an epitope specific to MCM10 immobilized on solid support. The epitope recognized by A300-131A maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CNA43, DNA43, homolog of yeast MCM10, hsMCM10, MCM10 minichromosome maintenance deficient 10, minichromosome maintenance complex component 10, PRO2249, protein MCM10 homolog
between 600 and 650
2 - 8°C
Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody A300-131A (lot A300-131A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MCM10 by western blot following immunoprecipitation. Sample: HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT 116 cells (not shown). Antibody: Affinity purified rabbit anti-MCM10 A300-131A used at 1 µg/mg lysate for IP and 0.5 µg/ml for WB. Detection: Chemiluminescence.