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Rabbit anti-MCM10 Antibody, Affinity Purified

Catalogue number:
A300-131A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to MCM10 immobilized on solid support. The epitope recognized by A300-131A maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CNA43, DNA43, homolog of yeast MCM10, hsMCM10, MCM10 minichromosome maintenance deficient 10, minichromosome maintenance complex component 10, PRO2249, protein MCM10 homolog

Species Reactivity:

Human

Immunogen:

between 600 and 650

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human MCM10 by western blot

Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody A300-131A (lot A300-131A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human MCM10 by western blot

Detection of human MCM10 by western blot following immunoprecipitation. Sample: HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT 116 cells (not shown). Antibody: Affinity purified rabbit anti-MCM10 A300-131A used at 1 µg/mg lysate for IP and 0.5 µg/ml for WB. Detection: Chemiluminescence.