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Rabbit anti-MCM10 Antibody, Affinity Purified - A300-131A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MCM10 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-131A
Name:
Rabbit anti-MCM10 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MCM10 immobilized on solid support. The epitope recognized by A300-131A maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CNA43, DNA43, homolog of yeast MCM10, hsMCM10, MCM10 minichromosome maintenance deficient 10, minichromosome maintenance complex component 10, PRO2249, protein MCM10 homolog
Species Reactivity:
Human
Immunogen:
Between 600 and 650
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody A300-131A (lot A300-131A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human MCM10 by western blot following immunoprecipitation. Sample: HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT 116 cells (not shown). Antibody: Affinity purified rabbit anti-MCM10 A300-131A used at 1 µg/mg lysate for IP and 0.5 µg/ml for WB. Detection: Chemiluminescence.

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