Antibody was affinity purified using an epitope specific to MCM6 immobilized on solid support. The epitope recognized by A300-127A maps to a region between residue 775 and the C-terminus (residue 821) of human Minichromosome Maintenance 6 using the numbering given in entry NP_005906.2 (GeneID 4175). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA replication licensing factor MCM6, MCG40308, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), minichromosome maintenance deficient (mis5, S. pombe) 6, Mis5, P105MCM
between 775 and C-term
2 - 8°C
Detection of human MCM6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody A300-127A (lot A300-127A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MCM6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 (Cat. No. A300-127A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB