Antibody was affinity purified using an epitope specific to MCM4 immobilized on solid support. The epitope recognized by A300-125A maps to a region between residues 325 and 375 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM4, hCdc21, homolog of S. pombe cell devision cycle 21, IMD54, minichromosome maintenance deficient 4, NKCD, NKGCD, P1-CDC21
between 325 and 375
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human MCM4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM4 antibody A300-125A (lot A300-125A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.