Antibody was affinity purified using an epitope from human SETDB1immobilized on solid support. The epitope recognized by A300-121A maps to a region between residues 1125 and 1175 of human SET Domain, Bifurcated 1 using the numbering given in entry NP_036564.2 (GeneID 9869). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ERG-associated protein with a SET domain, ESET, ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, H3-K9-HMTase4, histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 speci
between 1125 and 1175
2 - 8°C
Detection of human SETDB1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SETDB1 antibody A300-121A (lot A300-121A-3) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.