Antibody was affinity purified using an epitope from human DHX15 immobilized on solid support. The epitope recognized by A300-118A maps to a region between residues 150 and 200 using the numbering given in entry NP_001349.1 (GeneID 1665). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase #46, DBP1, DDX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAD/H box-15, DEAH (Asp-Glu-Ala-His) box helicase 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, DEAH box protein 15, HRH2, pre-mRNA-splicing factor ATP-depende
between 150 and 200
2 - 8°C
Detection of human DHX15 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody A300-118A (lot A300-118A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.