Antibody was affinity purified using a synthetic peptide representing phosphorylation at Serine 41 and surrounding residues of MCM2 immobilized on solid support. Immunogen for A300-117A was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 41 according to the numbering given in entry NP_0045117.2 (GeneID 4171). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BM28, CCNL1, cdc19, CDCL1, cell devision cycle-like 1, cyclin-like 1, D3S3194, DFNA70, DNA replication licensing factor MCM2, minichromosome maintenance deficient 2 (mitotin), minichromosome maintenance protein 2 homolog, MITOTIN, nuclear protein BM28
surrounding serine 41
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Phospho MCM2 (S41) by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho MCM2 (S41) antibody A300-117A (lot A300-117A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human and mouse MCM2 (Ser41) by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM2 (Ser41) (Cat. No. A300-117A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human Phospho MCM2 (Ser41) by western blot. Samples: Whole cell lysate (30 µg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: Affinity purified rabbit anti-phospho MCM2 (Ser41) antibody A300-117A used at 0.1 µg/ml. After probing with the phospho-specific antibodies, rabbit anti-MCM2 antibody A300-191A was used at 0.2 µg/ml to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.