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Rabbit anti-BLM Antibody, Affinity Purified - A300-110A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BLM Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-110A
Name:
Rabbit anti-BLM Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to BLM immobilized on solid support. The epitope recognized by A300-110A maps to a region between residue 1375 and the C-terminus (residue 1417) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Bloom syndrome protein, Bloom syndrome RecQ like helicase, Bloom syndrome, RecQ helicase-like, BS, DNA helicase, RecQ-like type 2, MGRISCE1, recQ protein-like 3, RECQ2, RECQL2, RECQL3
Species Reactivity:
Human, Mouse
Immunogen:
Between 1375 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BLM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse BLM by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-2) used for WB at 2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-110A (lot A300-110A-3) used for IP at 6 µg/mg lysate. BLM was also immunoprecipitated by a previous lot of this antibody (lot A300-110A-2) and A300-120A. For blotting immunoprecipitated BLM, A300-110A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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