Rabbit anti-ASH2 Antibody, Affinity Purified - A300-107A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support. The epitope recognized by A300-107A maps to a region between residue 50 and 100 of human Absent, Small, or Homeotic-Like 2 using the numbering given in entry NP_004665.1 (GeneID 9070).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody A300-107A (lot A300-107A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human ASH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody A300-107A (lot A300-107A-2) used for IP at 6 µg per reaction. ASH2 was also immunoprecipitated by rabbit anti-ASH2 antibody BL765. For blotting immunoprecipitated ASH2, BL765 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human ASH2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2 (Cat. No. A300-107A Lot 2) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB
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