Antibody was affinity purified using a phospho epitope specific to serine 178 of Paxillin immobilized on solid support. The epitope recognized by A300-100A maps surrounding serine 178 of human Paxillin using the numbering given in entry NP_002850.1 (GeneID 5829) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
paxillin, testicular tissue protein Li 134
surrounding serine 178
2 - 8°C
Detection of mouse phospho Paxillin (Ser178) by western blot. Samples: Whole cell lysate (approximately 2.4 x 10^5 cells/lane) from NIH 3T3 cells that were serum starved for 16 hours and then grown in suspension culture or plated on fibronectin (50 µg/ml). Whole cell lysate from cells grown on fibronectin was untreated or treated with lambda phosphatase. Antibody: Affinity purified rabbit anti-phospho Paxillin (Ser178) antibody A300-100A used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.