Antibody was affinity purified using an epitope specific to PARC/H7-AP1 immobilized on solid support. The epitope recognized by A300-098A maps to a region between residue 2475 and the C-terminus (residue 2527) of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CUL-9, cullin-9, H7AP1, p53-associated parkin-like cytoplasmic protein, PARC, parkin-like cytoplasmic p53 binding protein, UbcH7-associated protein 1
between 2475 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human PARC by western blot and immunoprecipitation. Samples: A) Whole cell lysate (100 µg) from untreated U2OS cells or U2OS derived cells that stably express a short hairpin RNA (shPARC) against PARC. B) Whole cell lysate (200 µg for input; 1 mg for IP) from BJAB cells. Antibodies: Affinity purified rabbit anti-PARC antibody A300-098A used at 0.04 µg/ml for WB (A & B) and at 2 µg/mg lysate for IP. PARC was also immunoprecipitated using A300-096A at 2 µg/mg lysate. Detection: Chemiluminescence with an exposure time of less than 5 minutes.