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Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified

Catalogue number:
A300-097A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PARC/H7-AP1 immobilized on solid support. The epitope recognized by A300-097A maps to a region between residues 900 and 950 of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CUL-9, cullin-9, H7AP1, p53-associated parkin-like cytoplasmic protein, PARC, parkin-like cytoplasmic p53 binding protein, UbcH7-associated protein 1

Species Reactivity:

Human

Immunogen:

between 900 and 950

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PARC/H7-AP1 by wester

Detection of human PARC/H7-AP1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody A300-097A (lot A300-097A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PARC/H7-AP1 by wester

Detection of human PARC/H7-AP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody A300-097A (lot A300-097A-3) used for IP at 6 µg per reaction. PARC/H7-AP1 was also immunoprecipitated by rabbit anti-PARC/H7-AP1 antibody A300-096A. For blotting immunoprecipitated PARC/H7-AP1, A300-097A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.