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Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified - A300-096A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-096A
Name:
Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PARC/H7-AP1 immobilized on solid support. The epitope recognized by A300-096A maps to a region between residues 1 and 50 of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CUL-9, cullin-9, H7AP1, p53-associated parkin-like cytoplasmic protein, PARC, parkin-like cytoplasmic p53 binding protein, UbcH7-associated protein 1
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human PARC/H7-AP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody A300-096A (lot A300-096A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human PARC/H7-AP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody A300-096A (lot A300-096A-2) used for IP at 6 µg per reaction. PARC/H7-AP1 was also immunoprecipitated by a previous lot of this antibody A300-096A-1. For blotting immunoprecipitated PARC/H7-AP1, A300-096A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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