Antibody was affinity purified using an epitope specific to mouse BID immobilized on solid support. The epitope recognized by A300-084A maps to a region between residues 1 and 50 of mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
2700049M22Rik, AI875481, AU022477, BH3-interacting domain death agonist, BID, p22 BID
between 1 and 50
2 - 8°C
Detection of mouse BID by western blot. Sample: Whole cell lysate (40 µg) from mouse embryonic fibroblast (MEF) cells. Antibody: Affinity purified rabbit anti-mouse BID antibody A300-084A used for WB at 1 µg/ml. Detection: Chemiluminescence.