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Rabbit anti-H2AX Antibody, Affinity Purified

Catalogue number:
A300-083A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to H2AX immobilized on solid support. The epitope recongized by A300-083A maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IHC
Product Type:

Antibodies

Alternative Names:

H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX

Species Reactivity:

Human, Mouse

Immunogen:

C-terminus

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human H2AX by western blot.

Detection of human H2AX by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse H2AX by western blot.

Detection of mouse H2AX by western blot. Samples: Whole cell lysate (15 µg) from NIH 3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human H2AX by immunohistoch

Detection of human H2AX by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (Cat. No. A300-083A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB