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Rabbit anti-H2AX Antibody, Affinity Purified

Catalogue number:
A300-082A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to H2AX immobilized on solid support. The epitope recognized by A300-082A maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, ICC-IF
Product Type:

Antibodies

Alternative Names:

H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX

Species Reactivity:

Human, Mouse

Immunogen:

C-terminus

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Histone H2A

Detection of human and mouse Histone H2AX by western blot. Samples: Nuclear extract from human HEK293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody A300-082A used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse H2AX by immunocytoche

Detection of mouse H2AX by immunocytochemistry. Samples: Asynchronous wild-type (+/+) and H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody A300-082A used at 2 µg/ml. Detection: Rhodamine labelled goat anti-rabbit IgG.