Antibody was affinity purified using an epitope specific to H2AX immobilized on solid support. The epitope recognized by A300-082A maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX
2 - 8°C
Detection of human and mouse Histone H2AX by western blot. Samples: Nuclear extract from human HEK293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody A300-082A used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of mouse H2AX by immunocytochemistry. Samples: Asynchronous wild-type (+/+) and H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody A300-082A used at 2 µg/ml. Detection: Rhodamine labelled goat anti-rabbit IgG.