Rabbit anti-H2AX Antibody, Affinity Purified
Antibody was affinity purified using an epitope specific to H2AX immobilized on solid support. The epitope recognized by A300-082A maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Data sheet/protocol: View or download
- MSDS: View or download
- Applications: WB, ICC-IF
Antibodies
H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX
Human, Mouse
C-terminus
2 - 8°C
Rabbit
Whole IgG
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Polyclonal
12352203
Detection of human and mouse Histone H2AX by western blot. Samples: Nuclear extract from human HEK293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody A300-082A used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of 10 seconds.
