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Rabbit anti-gamma-H2AX Antibody, Affinity Purified - A300-081A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-gamma-H2AX Antibody, Affinity Purified
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Size: 100 µg
£322.00
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Catalogue number:
A300-081A
Name:
Rabbit anti-gamma-H2AX Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to gamma-H2AX immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The epitope recongized by A300-081A maps to a region surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (GeneID 3014).

Applications:

WB, IHC, ICC-IF

Product Type:
Antibodies
Alternative Names:
H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX
Species Reactivity:
Human, Mouse
Immunogen:
Surrounding serine 140
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (15 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody A300-081A (lot A300-081A-19) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- gamma-H2AX (Cat. No. A300-081A Lot19) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB

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Detection of human gamma-H2AX by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purified rabbit anti-gamma-H2AX (Cat. No. A300-081A Lot12) used at a dilution of 1:5,000 (0.2µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.

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Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti- gamma-H2AX (Cat. No. A300-081A Lot19) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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