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Rabbit anti-CDC25a Antibody, Affinity Purified - A300-075A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CDC25a Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-075A
Name:
Rabbit anti-CDC25a Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CDC25a immobilized on solid support. The epitope recognized by A300-075A maps to a region between residues 175 and 225 of human Cell Division Cycle 25A using the numbering given in entry NP_001780.2 (GeneID 993). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CDC25 isoform A1-CAG, CDC25A2, CDC25A2-CAG isoform, dual specificity phosphatase CDC25A, M-phase inducer phosphatase 1
Species Reactivity:
Human
Immunogen:
between 175 and 225
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody A300-075A (lot A300-075A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human CDC25a by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody A300-075A (lot A300-075A-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody A300-076A. For blotting immunoprecipitated CDC25a, A300-075A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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