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Rabbit anti-Aurora A Antibody, Affinity Purified - A300-071A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Aurora A Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-071A
Name:
Rabbit anti-Aurora A Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Aurora A immobilized on solid support. The epitope recognized by A300-071A maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
AIK, ARK1, ARK-1, AURA, aurora 2, aurora kinase A, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, Aurora-related kinase 1, breast tumor-amplified kinase, BTAK, hARK1, PPP1R47, protein phosphatase 1, regulatory subunit 47, serine/threonine protein
Species Reactivity:
Human
Immunogen:
Between 75 and 125
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Aurora A by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody A300-071A (lot A300-071A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody A300-071A (lot A300-071A-2) used for IP at 3 µg per reaction. Aurora A was also immunoprecipitated by rabbit anti-Aurora A antibody A300-070A. For blotting immunoprecipitated Aurora A, A300-071A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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