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Rabbit anti-Aurora A Antibody, Affinity Purified

Catalogue number:
A300-071A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Aurora A immobilized on solid support. The epitope recognized by A300-071A maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

AIK, ARK1, ARK-1, AURA, aurora 2, aurora kinase A, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, Aurora-related kinase 1, breast tumor-amplified kinase, BTAK, hARK1, PPP1R47, protein phosphatase 1, regulatory subunit 47, serine/threonine protein

Species Reactivity:

Human

Immunogen:

between 75 and 125

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Aurora A by western b

Detection of human Aurora A by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody A300-071A (lot A300-071A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Aurora A by western b

Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody A300-071A (lot A300-071A-2) used for IP at 3 µg per reaction. Aurora A was also immunoprecipitated by rabbit anti-Aurora A antibody A300-070A. For blotting immunoprecipitated Aurora A, A300-071A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.