Antibody was affinity purified using an epitope specific to Aurora A immobilized on solid support. The epitope recognized by A300-070A maps to a region between residues 50 and 100 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
AIK, ARK1, ARK-1, AURA, aurora 2, aurora kinase A, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, Aurora-related kinase 1, breast tumor-amplified kinase, BTAK, hARK1, PPP1R47, protein phosphatase 1, regulatory subunit 47, serine/threonine protein
between 50 and 100
2 - 8°C
immunoprecipitation of human (Aurora A). Sample: 35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. Antibody: Affinity purified rabbit anti-Aurora A antibody A300-070A used at 4 µg/ml for IP. Detection: Autoradiography.