Antibody was affinity purified using an epitope specific to SMC4 immobilized on solid support. The epitope recognized by A300-064A maps to a region between residue 1225 and the C-terminus (residue 1288) of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPC, CAP-C, chromosome-associated polypeptide C, hCAP-C, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes protein 4, XCAP-C homolog
between 1225 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human SMC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (90 µg for WB; 0.5 mg for IP/WB) from HEK293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 A300-064A used at 0.2 or 0.1 µg/ml for WB and 5 µg for IP. Detection: Chemiluminescence with the indicated exposure times.