Rabbit anti-SMC4 Antibody, Affinity Purified - A300-064A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SMC4 immobilized on solid support. The epitope recognized by A300-064A maps to a region between residue 1225 and the C-terminus (residue 1288) of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, ICC
Detection of human SMC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (90 µg for WB; 0.5 mg for IP/WB) from HEK293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 A300-064A used at 0.2 or 0.1 µg/ml for WB and 5 µg for IP. Detection: Chemiluminescence with the indicated exposure times.
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