Antibody was affinity purified using an epitope specific to SMC4 immobilized on solid support. The epitope recognized by A300-063A maps to a region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPC, CAP-C, chromosome-associated polypeptide C, hCAP-C, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes protein 4, XCAP-C homolog
between 600 and 650
2 - 8°C
Detection of human SMC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody A300-063A (lot A300-063A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Localization of SMC4 to Mitotic Chromosomes by immunocytochemistry. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody A300-063A used at 5 µg/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.