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Rabbit anti-SMC4 Antibody, Affinity Purified - A300-063A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SMC4 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-063A
Name:
Rabbit anti-SMC4 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to SMC4 immobilized on solid support. The epitope recognized by A300-063A maps to a region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, ICC-IF

Product Type:
Antibodies
Alternative Names:
CAPC, CAP-C, chromosome-associated polypeptide C, hCAP-C, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes protein 4, XCAP-C homolog
Species Reactivity:
Human
Immunogen:
Between 600 and 650
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human SMC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody A300-063A (lot A300-063A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Localization of SMC4 to Mitotic Chromosomes by immunocytochemistry. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody A300-063A used at 5 µg/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.

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