Antibody was affinity purified using a phosphorylated epitope corresponding to phosphorylation at Serine 383 immobilized on solid support. Immunogen for A300-061A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 3 (GeneID 9126) around serine 383 according to the numbering given in entry NP_005436.1. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAM, Bamacan, basement membrane-associated chondroitin proteoglycan, BMH, CDLS3, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), chromosome-associated polypeptide, CSPG6, HCAP, SMC protein 3, SMC-3, SMC3L1, structural mai
surrounding serine 383
2 - 8°C
immunoprecipitation of human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 µg) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 A300-061A was used at 4 µg/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 A300-060A was used at 4 µg/mg lysate for IP and at 0.5 µg/ml for WB. Detection: Chemiluminescence.