Rabbit anti-SMC3 Antibody, Affinity Purified - A300-060A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SMC3 immobilized on solid support. The epitope recognized by A300-060A maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse SMC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for IP at 3 µg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot A300-060A-8). For blotting immunoprecipitated SMC3, A300-060A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SMC3 Cat. No. A300-060A Lot9 used at 1:1,000 (1µg/ml). Detection: DAB
Detection of mouse SMC3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SMC3 Cat. No. A300-060A Lot9 used at 1:1,000 (1µg/ml). Detection: DAB
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