Antibody was affinity purified using an epitope specific to SMC3 immobilized on solid support. The epitope recognized by A300-060A maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAM, Bamacan, basement membrane-associated chondroitin proteoglycan, BMH, CDLS3, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), chromosome-associated polypeptide, CSPG6, HCAP, SMC protein 3, SMC-3, SMC3L1, structural mai
between 1175 and C-term
2 - 8°C
Detection of human and mouse SMC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody A300-060A (lot A300-060A-9) used for IP at 3 µg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot A300-060A-8). For blotting immunoprecipitated SMC3, A300-060A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.