Antibody was affinity purified using an epitope specific to SMC2 immobilized on solid support. The epitope recognized by A300-057A maps to a region between residues 950 and 1000 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPE, CAP-E, chromosome-associated protein E, hCAP-E, SMC protein 2, SMC-2, SMC2 (structural maintenance of chromosomes 2, yeast)-like 1, SMC2L1, structural maintenance of chromosomes (SMC) family member, chromosome-associated protein E, structural mainte
between 950 and 1000
2 - 8°C
Detection of human SMC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody A300-057A (lot A300-057A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SMC2 by western blot. Samples: Whole cell lysate (90 µg) from mock transfected (E) or SMC2 transfected (T) HEKHEK293T cells. Antibody: Affinity purified rabbit anti-SMC2 A300-057A used at 0.1 µg/ml. Detection: Chemiluminescence with a 10 minute exposure.