Rabbit anti-SMC1 Antibody, Affinity Purified - A300-055A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SMC1 immobilized on solid support. The epitope recognized by A300-055A maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2 (GeneID 8243). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for IP at 6 µg per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018). For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 (Cat. No. A300-055A Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 (Cat. No. A300-055A Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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