*

Rabbit anti-SMC1 Antibody, Affinity Purified

Catalogue number:
A300-055A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to SMC1 immobilized on solid support. The epitope recognized by A300-055A maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2 (GeneID 8243). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan

Species Reactivity:

Human, Mouse

Immunogen:

between 1175 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse SMC1 by wes

Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human SMC1 by western blot

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for IP at 6 µg per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018). For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.

Detection of human SMC1 by immunohistoch

Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 (Cat. No. A300-055A Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

Detection of mouse SMC1 by immunohistoch

Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 (Cat. No. A300-055A Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB