Antibody was affinity purified using an epitope specific to SMC1 immobilized on solid support. The epitope recognized by A300-055A maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2 (GeneID 8243). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan
between 1175 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody A300-055A (lot A300-055A-6) used for IP at 6 µg per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018). For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.