Antibody was affinity purified using an epitope specific to Topo II Alpha immobilized on solid support. The epitope recognized by A300-054A maps to a region between residue 1500 and the C-terminus (residue 1531) of human Topoisomerase (DNA) II Alpha using the numbering given in entry NP_001058.2 (GeneID 7153). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA gyrase, DNA topoisomerase (ATP-hydrolyzing), DNA topoisomerase 2-alpha, DNA topoisomerase II, 170 kD, DNA topoisomerase II, alpha isozyme, TOP2, topoisomerase (DNA) II alpha 170kDa, TP2A
between 1500 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Topo II Alpha by western blot and immunoprecipitation. Samples: Whole cell lysate (25 µg/lane for A; 0.5 mg for IP in B; 25 µg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody A300-054A used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).