homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
between 1375 and 1425
2 - 8°C
Detection of human MDC1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody A300-053A (Lot A300-053A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MDC1 was also immunoprecipitated by Lot A300-053A-1 of rabbit anti-MDC1 antibody A300-053A. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).