Rabbit anti-MDC1 Antibody, Affinity Purified
Antibody was affinity purified using an epitope specific to MDC1 immobilized on solid support. The epitope recognized by A300-053A maps to a region between residues 1375 and 1425 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Antibodies
homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
Human
between 1375 and 1425
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human MDC1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody A300-053A (Lot A300-053A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MDC1 was also immunoprecipitated by Lot A300-053A-1 of rabbit anti-MDC1 antibody A300-053A. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
