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Rabbit anti-MDC1 Antibody, Affinity Purified

Catalogue number:
100 µg
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Antibody was affinity purified using an epitope specific to MDC1immobilized on solid support. The epitope recognized by A300-052A maps to a region between residues 925 and 975 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:


Alternative Names:

homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1

Species Reactivity:



between 925 and 975

Storage Temp:

2 - 8°C

Antibody Host:


Antibody Isotype:

Whole IgG

Antibody Type:




Detection of human MDC1 by western blot

Detection of human MDC1 by western blot and Immuno-precipitation. Samples: A) Nuclear extract (50 µg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody A300-052A used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies A300-052A and A300-053A used at 3.3 µg/mg lysate for IP followed by WB using A300-051A at 0.1 µg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

Detection of human MDC1 by immunohistoch

Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MDC1 (Cat. No. A300-052A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB