Antibody was affinity purified using an epitope specific to MDC1immobilized on solid support. The epitope recognized by A300-052A maps to a region between residues 925 and 975 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
between 925 and 975
2 - 8°C
Detection of human MDC1 by western blot and Immuno-precipitation. Samples: A) Nuclear extract (50 µg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody A300-052A used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies A300-052A and A300-053A used at 3.3 µg/mg lysate for IP followed by WB using A300-051A at 0.1 µg/ml. Detection: Chemiluminescence with exposure times less than 5 min.