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Rabbit anti-MDC1 Antibody, Affinity Purified - A300-052A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MDC1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-052A
Name:
Rabbit anti-MDC1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MDC1immobilized on solid support. The epitope recognized by A300-052A maps to a region between residues 925 and 975 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
Species Reactivity:
Human
Immunogen:
Between 925 and 975
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MDC1 by western blot and Immuno-precipitation. Samples: A) Nuclear extract (50 µg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody A300-052A used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies A300-052A and A300-053A used at 3.3 µg/mg lysate for IP followed by WB using A300-051A at 0.1 µg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

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Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MDC1 (Cat. No. A300-052A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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