Antibody was affinity purified using an epitope specific to DNMT1 immobilized on solid support. The epitope recognized by A300-041A maps to a region between residue 350 and 400 of human DNA-Methyltranserase 1 using the numbering given in entry NP_001370.1 (GeneID 1786). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ADCADN, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, Dnmt1, HSN1E, m.HsaI, MCMT
between 350 and 400
2 - 8°C
Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody A300-041A (lot A300-041A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DNMT1 by western blot and immunoprecipitation. Samples: Nuclear extract [50 µg/lane for A; 10 µg (input) or 1 mg (A300-042A IP) for B] from HeLa cells. Antibodies: A. Affinity purified rabbit anti-DNMT1 antibody A300-041A used at the indicated concentrations for WB. B. DNMT1 immunoprecipitated with affinity purified rabbit anti-DNMT1 antibody A300-042A then detected with affinity purified rabbit anti-DNMT1 antibody A300-041A used at 0.2 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 2 seconds.