Rabbit anti-DNMT1 Antibody, Affinity Purified - A300-041A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to DNMT1 immobilized on solid support. The epitope recognized by A300-041A maps to a region between residue 350 and 400 of human DNA-Methyltranserase 1 using the numbering given in entry NP_001370.1 (GeneID 1786). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody A300-041A (lot A300-041A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DNMT1 by western blot and immunoprecipitation. Samples: Nuclear extract [50 µg/lane for A; 10 µg (input) or 1 mg (A300-042A IP) for B] from HeLa cells. Antibodies: A. Affinity purified rabbit anti-DNMT1 antibody A300-041A used at the indicated concentrations for WB. B. DNMT1 immunoprecipitated with affinity purified rabbit anti-DNMT1 antibody A300-042A then detected with affinity purified rabbit anti-DNMT1 antibody A300-041A used at 0.2 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 2 seconds.
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