Goat anti-RENT1 Antibody, Affinity Purified - A300-036A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to RENT1 immobilized on solid support. The epitope recognized by A300-036A maps to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB
Detection of human and mouse RENT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody A300-036A (lot A300-036A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RENT-1 by western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immortalized B-cell from a normal individual). Antibody: Affinity purified goat anti-RENT-1 antibody A300-036A used at 1 µg/ml. Detection: Chemiluminescence.
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