Antibody was affinity purified using an epitope specific to USP7 immobilized on solid support. The epitope recognized by A300-034A maps to a region between residue 1050 and the C-terminus (residue 1102) of human Ubiquitin Specific Peptidase 7 using the numbering given in entry NP_003461.1 (GeneID 7874). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 7, HAUSP, Herpes virus-associated ubiquitin-specific protease, Herpesvirus-associated ubiquitin-specific protease, TEF1, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin s
Human, Mouse, Rat
between 1050 and C-term
2 - 8°C
Detection of mouse and rat USP7 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP7 antibody A300-034A (lot A300-034A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.