*

Rabbit anti-USP7 Antibody, Affinity Purified

Catalogue number:
A300-033A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to USP7 immobilized on solid support. The epitope recognized by A300-033A maps to a region between residues 1 and 50 of human Ubiquitin Specific Peptidase 7 using the numbering given in entry NP_003461.1 (GeneID 7874). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 eq5als 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

deubiquitinating enzyme 7, HAUSP, Herpes virus-associated ubiquitin-specific protease, Herpesvirus-associated ubiquitin-specific protease, TEF1, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin s

Species Reactivity:

Human, Mouse

Immunogen:

Between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse USP7 by wes

Detection of human and mouse USP7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP7 antibody A300-033A (lot A300-033A-8) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human USP7 by western blot

Detection of human USP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP7 antibody A300-033A (lot A300-033A-8) used for IP at 6 µg per reaction. USP7 was also immunoprecipitated by a previous lot of this antibody (lot A300-033A-7) and rabbit anti-USP7 antibody A300-034A For blotting immunoprecipitated USP7, A300-033A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human USP7 by immunohistoch

Detection of human USP7 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti-USP7 Cat. No. A300-033A Lot7 (left) and Lot8 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of mouse USP7 by immunohistoch

Detection of mouse USP7 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-USP7 Cat. No. A300-033A Lot7 (left) and Lot8 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB