Antibody was affinity purified using an epitope specific to USP7 immobilized on solid support. The epitope recognized by A300-033A maps to a region between residues 1 and 50 of human Ubiquitin Specific Peptidase 7 using the numbering given in entry NP_003461.1 (GeneID 7874). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 eq5als 1.0 mg of IgG.
deubiquitinating enzyme 7, HAUSP, Herpes virus-associated ubiquitin-specific protease, Herpesvirus-associated ubiquitin-specific protease, TEF1, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin s
Between 1 and 50
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse USP7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP7 antibody A300-033A (lot A300-033A-8) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human USP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP7 antibody A300-033A (lot A300-033A-8) used for IP at 6 µg per reaction. USP7 was also immunoprecipitated by a previous lot of this antibody (lot A300-033A-7) and rabbit anti-USP7 antibody A300-034A For blotting immunoprecipitated USP7, A300-033A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.