Antibody was affinity purified using an epitope specific to KIAA0460 immobilized on solid support. The epitope recognized by A300-031A maps to a region between residue 700 and 750 of human KIAA0460 using the numbering given in entry CAH70314.1 (GeneID 23248). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HSPC099, KIAA0460, regulation of nuclear pre-mRNA domain-containing protein 2
between 700 and 750
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human KIAA0460 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-KIAA0460 antibody A300-031A (lot A300-031A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.