Antibody was affinity purified using an epitope specific to MSH6 immobilized on solid support. The epitope recognized by A300-023A maps to a region between residue 1300 and the C-terminus (residue 1360) of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, mutS protein homolog 6, mutS-alpha 160 kDa subunit, p160, sperm-associated protein
between 1300 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human MSH6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-MSH6 antibody A300-023A (lot A300-023A-2) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.