Rabbit anti-MSH6 Antibody, Affinity Purified - A300-023A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to MSH6 immobilized on solid support. The epitope recognized by A300-023A maps to a region between residue 1300 and the C-terminus (residue 1360) of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human MSH6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-MSH6 antibody A300-023A (lot A300-023A-2) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MSH6 by immunohistochemistry. Sample: FFPE section of human testicular seminoma.Antibody: Affinity purified rabbit anti- MSH6 (Cat. No. A300-023A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB
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