Goat anti-MSH6 Antibody, Affinity Purified - A300-022A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to MSH6 immobilized on solid support. The epitope recognized by A300-022A maps to a region between residues 350 and 400 of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human MSH6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer.Antibody: Affinity purified goat anti-MSH6 antibody A300-022A (lot A300-022A-2) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MSH6 by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer.Antibody: Affinity purified rabbit anti-MSH6 (Cat. No. A300-022A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB
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