Rabbit anti-GTF2I/TFII-I Antibody, Affinity Purified - A300-018A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to GTF2I/TFII-I immobilized on solid support. The epitope recognized by A300-018A maps to a region between residue 950 and the C-terminus (residue 998) of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IHC
Detection of human GTF2I/TFII-I by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF2I/TFII-I antibody A300-018A (lot A300-018A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human GFT2I/TFII-I by immunohistochemistry. Sample: FFPE section of human breast carcinoma.Antibody: Affinity purified rabbit anti-GFT2I/TF II-I (Cat. No. A300-018A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
Detection of human GTF2I/TFII-I by western blot. Samples: Whole cell lysate (100 µg) from HEK293 cells treated with GTF2I/TFII-I siRNA (+) or untreated (-).Antibody: Affinity purified rabbit anti-GTF2I/TFII-I A300-018A used at 0.2 µg/ml. Detection: Chemiluminescence.
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