Antibody was affinity purified using an epitope specific to GTF2I/TFII-I immobilized on solid support. The epitope recognized by A300-018A maps to a region between residue 950 and the C-terminus (residue 998) of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAP135, BAP-135, Bruton tyrosine kinase-associated protein 135, BTKAP1, BTK-associated protein 135, BTK-associated protein, 135kD, DIWS, general transcription factor II-I, GTFII-I, IB291, SPIN, SRF-Phox1-interacting protein, TFII-I, WBS, WBSCR6, Williams-
between 950 and C-term
2 - 8°C
Detection of human GTF2I/TFII-I by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF2I/TFII-I antibody A300-018A (lot A300-018A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.