Antibody was affinity purified using an epitope specific to MLH1 immobilized on solid support.The epitope recognized by A300-015A maps to a region between residue 700 and the C-terminus (residue 756) of human mutL homolog 1 using the numbering given in entry NP_000240.1 (GeneID 4292).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COCA2, DNA mismatch repair protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, mutL homolog 1, colon cancer, nonpolyposis type 2, MutL protein homolog 1
Between 700 and C-term
2 - 8°C
Detection of human MLH1 by western blot. Sample: Nuclear extract (50 µg for WB; 1 mg for IP) from HeLa cells.Antibody: Affinity purified rabbit anti-MLH1 A300-015A used at 1 µg/ml for WB and 5 µg/mg lysate for IP. Detection: Chemiluminescence.