Rabbit anti-MLH1 Antibody, Affinity Purified

Catalogue number:

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A300-015A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µg _$$_

Product is available in:

N/A

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Applications:

WB, IP

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

Between 700 and C-term

Alternative Names:

COCA2, DNA mismatch repair protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, mutL homolog 1, colon cancer, nonpolyposis type 2, MutL protein homolog 1

Product Description:

Antibody was affinity purified using an epitope specific to MLH1 immobilized on solid support.The epitope recognized by A300-015A maps to a region between residue 700 and the C-terminus (residue 756) of human mutL homolog 1 using the numbering given in entry NP_000240.1 (GeneID 4292).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of human MLH1 by western blot. Sample: Nuclear extract (50 µg for WB; 1 mg for IP) from HeLa cells.Antibody: Affinity purified rabbit anti-MLH1 A300-015A used at 1 µg/ml for WB and 5 µg/mg lysate for IP. Detection: Chemiluminescence.

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