Antibody was affinity purified using an epitope specific to CSN2 immobilized on solid support. The epitope recognized by A300-012A maps to a region between residues 325 and 375 of human COP9 constitutive photomorphogenic homolog subunit 3 using the numbering given in entry NP_003644.2 (GeneID 8533). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COP9 complex subunit 3, COP9 constitutive photomorphogenic homolog subunit 3, COP9 signalosome complex subunit 3, CSN3, JAB1-containing signalosome subunit 3, SGN3, signalosome subunit 3
between 325 and 375
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human CSN3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody A300-012A (lot A300-012A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CSN3 by western blot. Samples: Whole cell lysate (50 µg - E; 25 µg - T) from HEK HEK293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).Antibody: Affinity purified rabbit anti-CSN3 antibody A300-012A used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.