Antibody was affinity purified using an epitope specific to MKLP1 immobilized on solid support. The epitope recognized by A300-010A maps to a region between residues 1 and 50 of human Mitotic Kinesin-Like 1 using the numbering given in entry NP_004847.2 (GeneID 9493). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CHO1, kinesin-like 5 (mitotic kinesin-like protein 1), Kinesin-like protein 5, kinesin-like protein KIF23, KNSL5, Mitotic kinesin-like protein 1, MKLP1, MKLP-1
between 1 and 50
2 - 8°C
Detection of human MKLP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with Nocodazole (+) or mock treated (-).Antibody: Affinity purified rabbit anti-MKLP1 antibody A300-010A (lot A300-010A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.