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Rabbit anti-BRCA2 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 26 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

BRCA2

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 3350 and C-term

Alternative Names:

FAD1; FANCD; Fanconi anemia group D1 protein; mutant BRCA2; PNCA2; XRCC11; FANCD1; FAD; truncated breast cancer 2; BROVCA2; breast cancer type 2 susceptibility protein; BRCA1/BRCA2-containing complex, subunit 2; breast cancer 2, early onset; GLM3; breast

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Product Description:

Rabbit anti-BRCA2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human BRCA4 by western blot
Detection of human BRCA4 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, OVCAR-3, Hep-G2, K-562, Jurkat, HeLa, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA4 antibody A300-005A (lot A300-005A-4) for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRCA2 by western blot
Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRCA2 antibody A300-005A (lot A300-005A-4) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot A300-005A-3). For blotting immunoprecipitated BRCA2, A300-005A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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