Antibody was affinity purified using an epitope specific to Phospho BRCA1 (S1524) immobilized on solid support. Immunogen for A300-001A was a phosphorylated synthetic peptide, which represented a portion of human Breast Cancer Gene 1 (GeneID 672) around serine 1524 according to the numbering given in entry NP_009225.1. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BRCA1/BRCA2-containing complex, subunit 1, BRCAI, BRCC1, breast and ovarian cancer susceptibility protein 1, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, BROVCA1, early onset breast cancer 1, Fanconi anemia, complementation g
surrounding serine 1524
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Phospho BRCA1 (S1524) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells either treated with etoposide (+) or mock treated (-).Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody A300-001A (lot A300-001A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.