Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody A190-222A used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.