Chicken anti-6-His Tag Antibody Affinity Purified
Supplier:
Catalogue number:
A190-212A
Size:
1 mg
Product is available in:
This product is no longer available to order.
Chickens were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. This antibody does not detect all N-terminal 6-His tagged proteins. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Product Type:
Antibodies
Storage Temp:
2 - 8°C
Antibody Host:
Chicken
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-6-His antibody A190-212A used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Antibodies from Bethyl Laboratories, Inc.: