Chicken anti-6-His Tag Antibody Affinity Purified - A190-212A - 1 mg - Bethyl Laboratories, Inc. - antibodies
Chickens were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. This antibody does not detect all N-terminal 6-His tagged proteins. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, ICC, ELISA
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-6-His antibody A190-212A used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
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