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Goat anti-Glu-Glu Tag Antibody Affinity Purified

Catalogue number:
Size:
1 mg _$$_
Product is available in:
  • UK
  • Ireland
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  • Rest of World
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Applications:

WB, IP, ICC, ELISA

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Product Description:

Goats were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of Glu-Glu-tagged Protein by w
Detection of Glu-Glu-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Glu-Glu antibody A190-210A used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
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