Chicken anti-ECS (DYKDDDDK) Tag Antibody, Affinity Purified
Supplier:
Catalogue number:
A190-200A
Size:
1 mg
Product is available in:
This product is no longer available to order.
Chickens were immunized with ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By ELISA and Western blot, this antibody reacts with FLAGTM.
Product Type:
Antibodies
Storage Temp:
2 - 8°C
Antibody Host:
Chicken
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
Detection of ECS (DDDK)-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-ECS (DDDK) antibody A190-200A used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.