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Goat anti-S Tag Antibody Biotinylated

Catalogue number:
Size:
0.1 mg _$$_
Product is available in:
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Applications:

WB, ELISA

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Product Description:

Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of S-Tag-tagged Protein by wes
Detection of S-Tag-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-S-Tag antibody A190-134B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 3 seconds.
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