Goat anti-V5 Tag Antibody Biotinylated

Catalogue number:

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A190-119B

Supplier:

Bethyl Laboratories, Inc.

Size:

0.1 mg _$$_

Product is available in:

N/A

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Applications:

WB, ELISA

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Data sheet: View or download
MSDS: View or download

Product Description:

Goats were immunized with a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-V5 antibody A190-119B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 3 seconds.

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