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Goat anti-V5 Antibody Biotinylated - A190-119B - 0.1 mg - Bethyl Laboratories, Inc. - antibodies

Goat anti-V5 Antibody Biotinylated
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Size: 0.1 mg
£190.00
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Catalogue number:
A190-119B
Name:
Goat anti-V5 Antibody Biotinylated
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Goats were immunized with a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin.Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, ELISA

Product Type:
Antibodies
Storage Temp:
2 - 8°C
Antibody Host:
Goat
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-V5 antibody A190-119B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 3 seconds.

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