Goat anti-T7 Antibody Biotinylated - A190-116B - 0.1 mg - Bethyl Laboratories, Inc. - antibodies
Goat anti-T7 Antibody Biotinylated
Catalogue number:
A190-116B
Name:
Goat anti-T7 Antibody Biotinylated
Supplier:
Availability:





Goats were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin.Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, ELISA
Product Type:
Antibodies
Storage Temp:
2 - 8°C
Antibody Host:
Goat
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203

Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-T7 antibody A190-116B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 10 seconds.
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